Analytical Techniques In DNA Sequencing pdf epub mobi txt 电子书下载 2026

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出版者:Marcel Dekker Inc

作者:Nunnally, Brian K. 编

出品人:

页数:256

译者:

出版时间:2005-6

价格:$ 282.44

装帧:HRD

isbn号码:9780824753429

丛书系列:

图书标签:

DNA sequencing
Analytical techniques
Molecular biology
Genomics
Biotechnology
Genetic analysis
Bioinformatics
Laboratory techniques
Life sciences
Research methods

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具体描述

While one marvels at the DNA generated by the Human Genome Project, perhaps just as significant and fascinating is the development of the amazing analytical technology that has permitted us entry into the genomic era and a whole new level of scientific understanding. "Analytical Techniques in DNA Sequencing" takes a look at the various DNA sequencing techniques that were developed and put to use during and after the Human Genome Project. Nunnally makes a special effort to trace the development of this branch of research since the early days of radioisotope sequencing. Special attention is paid to the breakthrough Sanger Method (1977), as well as to several other techniques that improved the accuracy, improved the detection limits, and in a variety of ways dramatically reduced the time needed to generate a DNA sequence.This comprehensive volume describes the improvements in DNA separation based on advanced capillary electrophoresis and microchip sequencing. It also looks at the use of mass spectrometry and single molecule detection, and takes an in-depth look at the use of various fluorescence methods for the detection of sequencing fragments. One chapter is dedicated to chip-based microfabricated sequencing systems, which could well be the future of DNA sequencing. This book's ten chapters, authored by a variety of leading experts in the field, include many of the far-reaching applications of DNA sequencing. Entire chapters are dedicated to DNA typing for forensics, a re-evaluation of ancient DNA, and genome analysis.

这本书旨在为读者提供对DNA测序技术的深入理解和全面解析，其核心目标是帮助读者掌握从基础概念到最新进展的知识体系。书中将以系统化的方式介绍分析技术的各类方法，包括传统的基于电泳与凝胶电泳的策略，以及现代高通量测序平台所采用的计算与算法支持的流程。在每一个章节，内容都围绕如何提取和解析生物样本中的遗传信息展开，从分子层面探讨不同技术在不同场景下的适用性。书中详细介绍了各类分析手段的原理、操作步骤及其优势与局限性，例如实时PCR在定量分析中的应用、测序数据处理过程中涉及的质量控制标准，以及如何通过生物信息学工具对得出的结果进行解读和验证。这些内容不仅涵盖了技术细节，还结合实际案例，帮助读者更好地理解这些方法在科研和工业中的具体运用。此外，书中对新兴分析技术的介绍颇丰富，探讨了单细胞测序、长读长测序及多组学整合等前沿方向。这些章节不仅展示了当前研究的发展趋势，也为未来技术进步提供了思考的方向。内容设计严谨，逻辑清晰，适合对生物信息学和基因测序领域有较深兴趣或基础知识的读者参考。通过全面系统的讲解，这本书不仅帮助读者理解DNA测序技术的基本原理，更强调了分析过程中的科学思维与实际操作技能，强化了对复杂数据的处理能力。这种内容设置旨在为从初学者到专家的不同层次用户提供有价值的参考资料。书中还特别注重理论与实践的结合，通过详细的实验步骤和案例分析，帮助读者掌握技术应用的具体技巧。每一章均配以图表、示意图及思维导图，使复杂信息更加直观易懂。同时，作者在写作中注重语言的简洁明了，避免使用过于专业或晦涩的术语，以便读者能迅速把握核心概念和要点。总体而言，这本书不仅是对DNA测序分析技术的全面介绍，更是一份指导性资料，帮助读者在不断发展的科学领域中找到正确的方向与方法，提升自己的专业素养。通过精心编写的内容，它为各类研究人员、学生和科研工作者提供了坚实的理论支撑和实际操作指南，是一本值得深入阅读的重要书籍。