PCR Mutation Detection Protocols pdf epub mobi txt 电子书下载 2025

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出版者:Humana Press

作者:Theophilus, Bimal D. M. (EDT)/ Theophilus, Bimal D. M./ Rapley, Ralph/ Rapley, Ralph (EDT)

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页数:224

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出版时间:2002-4-4

价格:USD 99.00

装帧:Hardcover

isbn号码:9780896036178

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Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.