"Guide to Yeast Genetics and Molecular Biology" presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. It covers: basic methods in yeast genetics; physical and genetic mapping; making and recovering mutants; cloning and Recombinant DNA methods; high-efficiency transformation; preparation of yeast artificial chromosome vectors; basic methods of cell biology; immunomicroscopy; protein targeting assays; biochemistry of gene expression; vectors for regulated expression; and, isolation of labeled and unlabeled DNA, RNA, and protein.
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